DEFINED CELL CULTURE MEDIA
DEFINED CELL CULTURE MEDIA is banned in cosmetics in the EU — Annex II, entry 167 of Regulation (EC) No 1223/2009. Review the regulatory details and Safety Gate alerts.
Regulatory data checked on 2026-09-05. The legal status follows Regulation (EC) No 1223/2009 and its annexes; the substance is identified from the European Commission's CosIng database.
About the ingredient
Defined Cell Culture Media is a cell culture phase consisting of: Sodium Chloride, Sodium Bicarbonate, Glucose, Sodium Acetate, Glutamine, Arginine, Potassium Chloride, calcium nitrate, Magnesium Sulfate, Sodium Phosphate, Magnesium Chloride, Leucine, Disodium Phosphate, Sodium Pyruvate, Valine, Serine, Adenine, Cystine, Lysine HCl, Proline, Histidine HCl, Hydroxyproline, Cysteine HCl, Histidine, Inositol, Threonine, Choline Chloride, Glycine, Asparagine, Glutamic Acid, Phenylalanine, Alanine, Methionine, Calcium Chloride, Tryptophan, Aspartic Acid,Tyrosine, Isoleucine, hypoxanthine, pyridoxal HCl, Glutathione, PABA, Folic Acid, Cyanocobalamin, Ferrous Sulfate, thymidine, Thiamine HCl, Calcium Pantothenate, Zinc Sulfate, Sodium Metasilicate, Linoleic Acid, ferric nitrate, Riboflavin, Niacinamide, Linoleic Acid, Pyridoxine HCl, Biotin, selenious acid, ammonium molybdate, ammonium vanadate, Copper Sulfate, Manganese Sulfate
EU regulatory status
Annex of Regulation (EC) No 1223/2009: II/167
What entry II/167 of the Regulation says
4-Aminobenzoic acid and its esters, with the free amino group
Source: the European Commission's official CosIng database and Regulation (EC) No 1223/2009 of the European Parliament and of the Council on cosmetic products. How we assess ingredients — full methodology.
Last updated:
Functions in cosmetics
Frequently asked questions
Is DEFINED CELL CULTURE MEDIA safe in cosmetics?
DEFINED CELL CULTURE MEDIA falls under Annex II to Regulation (EC) No 1223/2009, and under Article 14 a cosmetic product placed on the EU market must not contain a substance prohibited by that annex. This is a legal status, not a toxicity finding about any particular product. Where a non-compliant product is found, the competent authority requires the responsible person to bring it into conformity, withdraw it from the market or recall it, proportionate to the risk; products presenting a serious risk are also notified through the EU Safety Gate.
In which annex of Regulation 1223/2009 does DEFINED CELL CULTURE MEDIA fall?
DEFINED CELL CULTURE MEDIA falls under reference II/167 in the annexes of Regulation (EC) No 1223/2009 on cosmetic products. An annex entry may name a substance directly or cover it as part of a broader category. The first part (Roman numeral) indicates the annex: II – prohibited substances, III – substances restricted by conditions, IV – permitted colorants, V – permitted preservatives, VI – permitted UV filters.
What functions does DEFINED CELL CULTURE MEDIA perform in cosmetics?
According to the European Commission's CosIng database, DEFINED CELL CULTURE MEDIA performs the following functions in cosmetics: SKIN CONDITIONING. Functions are defined in the CosIng glossary and describe the technological role of a substance in the finished product.
Are there Safety Gate alerts concerning DEFINED CELL CULTURE MEDIA?
Our copy of the EU Safety Gate database currently contains no alerts linking DEFINED CELL CULTURE MEDIA to a cosmetic recall. The database is refreshed regularly from the European Commission's official Safety Gate system.