DEFINED CELL CULTURE MEDIA
DEFINED CELL CULTURE MEDIA is banned in cosmetics in the EU — Annex II, entry 167 of Regulation (EC) No 1223/2009. Review the regulatory details and Safety Gate alerts.
Regulatory status as of 2026-08-28 (per the CosIng database).
About the ingredient
Defined Cell Culture Media is a cell culture phase consisting of: Sodium Chloride, Sodium Bicarbonate, Glucose, Sodium Acetate, Glutamine, Arginine, Potassium Chloride, calcium nitrate, Magnesium Sulfate, Sodium Phosphate, Magnesium Chloride, Leucine, Disodium Phosphate, Sodium Pyruvate, Valine, Serine, Adenine, Cystine, Lysine HCl, Proline, Histidine HCl, Hydroxyproline, Cysteine HCl, Histidine, Inositol, Threonine, Choline Chloride, Glycine, Asparagine, Glutamic Acid, Phenylalanine, Alanine, Methionine, Calcium Chloride, Tryptophan, Aspartic Acid,Tyrosine, Isoleucine, hypoxanthine, pyridoxal HCl, Glutathione, PABA, Folic Acid, Cyanocobalamin, Ferrous Sulfate, thymidine, Thiamine HCl, Calcium Pantothenate, Zinc Sulfate, Sodium Metasilicate, Linoleic Acid, ferric nitrate, Riboflavin, Niacinamide, Linoleic Acid, Pyridoxine HCl, Biotin, selenious acid, ammonium molybdate, ammonium vanadate, Copper Sulfate, Manganese Sulfate
EU regulatory status
Annex of Regulation (EC) No 1223/2009: II/167
Source: the European Commission's official CosIng database and Regulation (EC) No 1223/2009 of the European Parliament and of the Council on cosmetic products. How we assess ingredients — full methodology.
- View the entry in the CosIng database (European Commission)
- Open Regulation 1223/2009 in EUR-Lex
- Check the chemical profile in PubChem
Last updated:
Functions in cosmetics
Frequently asked questions
Is DEFINED CELL CULTURE MEDIA safe in cosmetics?
DEFINED CELL CULTURE MEDIA is banned in the EU under Annex II of Regulation (EC) No 1223/2009 and may not be used in cosmetics placed on the EU market. Cosmetics containing this substance are withdrawn by national inspectorates and reported to the EU Safety Gate database.
In which annex of Regulation 1223/2009 is DEFINED CELL CULTURE MEDIA listed?
DEFINED CELL CULTURE MEDIA is listed under reference II/167 in the annexes of Regulation (EC) No 1223/2009 on cosmetic products. The first part (Roman numeral) indicates the annex: II – prohibited substances, III – substances restricted by conditions, IV – permitted colorants, V – permitted preservatives, VI – permitted UV filters.
What functions does DEFINED CELL CULTURE MEDIA perform in cosmetics?
According to the European Commission's CosIng database, DEFINED CELL CULTURE MEDIA performs the following functions in cosmetics: SKIN CONDITIONING. Functions are defined in the CosIng glossary and describe the technological role of a substance in the finished product.
Are there Safety Gate alerts concerning DEFINED CELL CULTURE MEDIA?
Our copy of the EU Safety Gate database currently contains no alerts linking DEFINED CELL CULTURE MEDIA to a cosmetic recall. The database is refreshed regularly from the European Commission's official Safety Gate system.